Fig. 4

（A） The number of autophagosomes and autolysosomes are compared between CLP and sham animals.All data are expressed as the mean±SD. Data were analyzed for statistical significance using the MannWhitney test. The increase in autolysosomes in the CLP group was statistically significant（*P＜0.05; n＝3）; the mean increases in autophagosomes in the CLP group compared to the sham group did not reach statistical significance. NS, not significant.（B） Electron microscopic images of the liver; a: Liver sample obtained from sham-operated mice. Organelles in the hepatocyte are generally intact, and lysosomes do not contain discrete membrane structures, although the non-homogeneous electron-dense material often seen in（hetero） lysosomes most certainly represents the end-stage degradation of phospholipids and other cytoplasmic materials（material at the light microscopic level referred to as lipofuscin）; b-e: CLP-operated mice. Double arrow heads indicate the complex structures bounded by two membranes（autophagosomes）; arrow heads indicate single membrane-bound lysosomal complexes with degraded organelle content（autolysosomes）; e: the double arrow head indicates an autophagosome that clearly contains a damaged mitochondrion.