Fig. 2

Expression of Nczf afterinduction of apoptosis by adriamycin:
（A） NIH3T3 cells were cultured in the presence of adriamycin（200ng/ml）. Cells were harvested at the indicated time and percentage of apoptotic cells were examined by FACS（left panel）. Percentage of apoptotic cells （AnnexinV^＋PI^－cells） was plotted（right panel）. Closed bar: without adriamycin, shaded bar: with adriamycin.
（B） RNA was isolated at each time point and Nczf mRNA expression was examined by reverse transcribed PCR（left panel） or qPCR （right panel）. The data are expressed as relative to the level of control（0hr）. Closed bar: without adriamycin, shaded bar: with adriamycin.