Fig. 3

Expression of Nczf after induction of apoptosis by dexamethasone:
（A） Murine thymocytes were cultured in the presence or absence of dexamethasone（100nM） for 5 hours and cells were collected. Percentage of apoptotic cells was examined by FACS.
（B） RNA was isolated at each time point and Nczf mRNA expression was examined by reverse transcribed PCR（left panel） or qPCR（right panel）. The data are expressed as relative to the level of control（0hr）. In（A） and （B）,（－） indicates without dexamethasone and （＋） indicates with dexamethasone.