Fig. 1

Identification and characterization of SP cells in HCC. (A) Flow cytometry analysis with Hoechst 33342 staining demonstrated that Huh7 cells included 0.25％ of SP cells. Only 1 × 10³ SP cells purified from Huh7 cells could develop tumors in the NOD/SCID xenograft transplant assay (B) Immunocytochemical analyses of BMI1 expression in SP and non-SP cells purified from Huh7 cells.