Fig. 1

JNJ-10198409 inhibits NB cell growth at nanomolar concentrations.
A, Chemical structure of JNJ-10198409.
B, JNJ-10198409 inhibits proliferation of NB cell lines SK-N-AS and IMR32 and a primary NB sphere-forming line NB153. EC₅₀ curves are shown demonstrating the effect of JNJ-10198409 on the proliferation of NB cell lines, using AlamarBlue as a read-out.（SK-NAS:EC₅₀＝20 nM, IMR32: EC₅₀＝10 nM, NB153: EC₅₀＝92 nM）
C, EC₅₀ curves are shown demonstrating the effect of JNJ-10198409 on the self-renewal and survival of cells from the bone marrow of high risk NB patients as measured in a sphere formation assay. NB cells（NB12 and NB88R2） had a dose-dependent decrease in sphere formation with EC₅₀ values in the nanomolar range（283 nM for NB12 and 68 nM for NB88R2） while normal pediatric neural crest-like stem cells（SKPs, line FS105） were unaffected. Points, mean of 2 independent experiments; Bars, SD.