Fig. 2

JNJ-10198409 induces apoptotic cell death of cells from NB patient’s bone marrow.
A, NB88R2 cells were treated with DMSO, 0.1 μM JNJ-10198409 or 1 μM JNJ-10198409 for 72 hours. Treated cells were deposited on slides by cytospin and subjected to immunostaining for Ki67（red） and cleaved caspase 3（green） with Hoechst 33342-staining of nuclei（blue）. A representative field is shown for the indicated treatments.
B, Quantification of live and apoptotic cells from 6 random fields for each treatment condition as described in Methods.
C, NB88R2 cells were treated with 0.1 μM JNJ-10198409 or DMSO for 48 hours. Immunoblots of JNJ-10198409-treated cell lysates demonstrated increased PARP cleavage. Total protein loading was visualized by p38MAPK immunuoblotting.