Fig. 3

JNJ-10198409 inhibits Src phosphorylation but does not alter PDGFR-dependent signal transduction in cells from NB patients.
A, Diagram of PDGF-dependent signal transduction pathways with selected PDGFR targets and interaction partners indicated.
B, NB88R2 or NIH-3T3 cells were pretreated with JNJ-10198409 and transient stimulation with PDGF as described in Methods. Immunoblots of NB88R2 cell lysates show no activation of PDGF-dependent signaling pathways while those for NIH-3T3 demonstrate PDGF-dependent stimulation of Shc, Akt, PLCγ and ERK1/2 phosphorylation that is blocked by JNJ-10198409 pretreatment of cells.
C, JNJ-10198409 treatment decreased c-Src phosphorylation in NB88R2 cells. Cells were treated with 0.1 μM JNJ-10198409 or DMSO for 48 hours, lysed, immunoprecipitated with anti-phosphotyrosine antibodies（4G10, top panel） or control IgG（middle panel） and immunoblotted for c-Src. Equal volume of input lysate was immunoblotted for c-Src（lower panel）