Fig. 10

Phosphorylation of SNAP-23 on Ser120 and Ser95 involved in IKK2-mediateddegranulation.

（A） Expression of phospho-mimetic mutant of SNAP-23 partially rescue the impairment of FceRI-induced β-hexosaminidase release in IKK2-/- mast cells. Mast cells were infected with retroviruses of pMX-puro-FLAGWT SANP-23, pMX-puro-FLAG-SNAP-23EE, or pMXpuro （Mock）. Cells were cross-linked upon FcɛRI.
Thirty minutes later, β-hexosaminidase activity in the supernatants and lysates of the cell pelletes was quantified.*P＜0.01. NS, not significant. （B） For passive cutaneous anaphylaxis, mock vector transduced WT mast cells, and mock vector, WT SNAP-23, or SNAP-23EE transduced IKK2-/- mast cells, were injected intradermally into the right ear of W/Wv mice. Four weeks later, PCA were induced in these mice. *P＜0.01. NS, not significant. （ref.3）