Fig. 5

Over-expression of IKK2 results in increase of degranulation.

RBL-2H3 cells （basophil line） were transfected with pcDNA3.1/Zeo GFP-IKK2, pcDNA3.1/Zeo GFPIKK2KM, and pcDNA3.1/Zeo GFP vector（ as control）.
Cells were cross-linked upon FcɛRI. Thirty minutes later, β-hexosaminidase activity in the supernatants and lysate of the cell pelletes was quantified. *P＜0.01. NS, not significant. （ref.3）