Fig. 6

NF-κB activation is not essential for mast cell degranulation.

（A） RBL-2H3 cells were transfected with pcDNA3.1/ Zeo IκBαM （dominant negative mutantof IkBα） and pcDNA3.1/Zeo mock vector（ as control）. Cells were transfected with HIV-3x-κB luciferase and β-galactosidase plasmid. 24 hours later, cells were stimulated with （black columns） or without （white columns） TNF-α. 6 hours later, luciferase activity was measured. *P＜0.01.（B） Cells were cross-linked upon FceRI. Thirty minutes later, β-hexosaminidase activity in the supernatants and lysate of the cell pelletes was quantified. （ref.3）