Fig. 9

IKK2 phosphorylates SNAP-23 on Ser120 and Ser95.

（A） Equal amounts of purified wild type and mutant GST-SNAP-23 proteins were incubated with ［γ-32P］ ATP and IKK2 and the reaction was analyzed by SDSPAGE and PhosphorImager analysis. （B） IgE-induced phosphorylation of SNAP-23 on Ser120 and Ser95 is impaired in IKK2-/- mast cells. WT or IKK2-/- mast cells were cross-linked upon FceRI. Fifteen minutes later, cell lysates were recovered and analyzed by immunoblot analysis using phosphorylation site specific antibodies. （ref.3）