Fig. 3

RT-PCR analysis for the demethylation study of OSCC-derived cell lines. After treatment with 5-AzaC, restoration of headpin mRNA expression was found in the 4 cell lines; SAS, HSC-3, OK-92 and SCC4, of 5 ones which showed down-regulation of the gene before the treatment. "-", before 5-AzaC treatment; "+", after 5-AzaC treatment; GAPDH, internal marker.