Fig. 3 The Image J software program was used to evaluate the 4 items in the black circle, which indicates an anastomotic site that was confirmed to have good blood flow.

①The staining start time was 22 seconds.
②The peak staining value（100） was achieved at 34 seconds
③The time from the start of staining to the peak of staining was 12 seconds
④Staining value at 5 seconds after the start of the staining, the staining value was 60.