Fig. 4 TGF-β (A, B) and IL-6 (C, D) gene expression in the main tumor. A, C, TGF-β (A) and IL-6 (C) mRNA levels in the main tumor of varying tumor sizes. Relative mRNA levels are represented as mean ± SEM. P-value was calculated by one-way ANOVA. *p＜0.05, **p＜0.01 by post-hoc test (Tukey-Kramer method) . B, D, Linear least-squares regression analysis between mRNA levels of RORC (Fig. 1) and TGF-β (A) or IL-6 (C) .