Fig. 5 Foxp3 (A, B) and IL-2 (C, D) gene expression in the main tumor. A, C, Foxp3 (A) and IL-2 (C) mRNA levels in the main tumor of varying tumor sizes. Relative mRNA levels are represented as mean ± SEM. P-value was calculated by one-way ANOVA. **p＜0.01 by post-hoc test (Tukey-Kramer method) . B, D, Linear least-squares regression analysis between mRNA levels of RORC (Fig. 1) and Foxp3 (B) or IL-2 (D) .